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目的:超声剪切波弹性成像及血流定量参数与乳腺癌组织GATA结合蛋白3（GATA binding protein 3，GATA3）和CD34表达水平的关系。方法:采用Resona R9全数字高端彩色多普勒超声诊断仪对患者乳腺进行超声剪切波弹性成像及血流参数检查，记录最大弹性值（maximum elasticity，Emax）、平均弹性值（mean elasticity，Emean）和最小弹性值（minimum elasticity，Emin）、搏动指数（pulsatility index，PI）、阻力指数（resistance index，RI）。采用免疫组织化学法检测乳腺癌组织和癌旁组织中的GATA3和CD34表达水平。结果:相较于癌旁正常组织，乳腺癌组织中GATA3低表达的比例显著升高，差异有统计学意义（ χ 2=9.67， P=0.002），CD34高表达的比例显著升高，差异有统计学意义（ χ 2=37.10， P<0.001）。GATA3高表达乳腺癌患者的Emax、Emin、PI、RI显著低于低表达患者，差异有统计学意义（ t=4.14、4.01、5.39、4.10， P<0.05）。CD34高表达乳腺癌患者的Emax、Emean、Emin、PI、RI显著高于低表达患者，差异有统计学意义（ t=4.55、4.04、5.49、3.89、5.27， P<0.05）。多因素二元Logistic回归分析结果显示，Emax、PI、RI是乳腺癌组织GATA3低表达的独立危险因素，差异有统计学意义[ OR=1.156（1.057~2.136）、1.243（1.031~2.054）、1.217（1.055~1.993）， P<0.05]；Emax、Emean、PI、RI是乳腺癌组织CD34高表达的独立危险因素，差异有统计学意义[ OR=1.176（1.048~1.934）、1.145（1.047~2.112）、1.213（1.065~2.004）、1.224（1.037~2.069）， P<0.05]。超声剪切波弹性成像联合血流定量参数评估乳腺癌组织GATA3低表达的灵敏度和特异度分别为78.7%和81.7%，评估乳腺癌组织CD34高表达的灵敏度和特异度分别为81.5%和76.6%。 结论:超声剪切波弹性成像及血流定量参数与乳腺癌组织GATA3和CD34表达水平有关，并有一定评估价值。

Objective:To investigate the relationship between ultrasonic shear wave elastic imaging, blood flow quantitative parameters and expression levels of GATA binding protein 3 (GATA3) and CD34 in breast cancer tissues.Methods:Ultrasound shear wave elastic imaging and blood flow parameter examination were performed with Resona R9 full-digital high-end color Doppler ultrasound diagnostic instrument of Shenzhen Mindray Biomedical Electronics Co., LTD., and maximum elasticity values (Emax), mean elasticity (Emean), minimum elasticity (Emin), pulsatility index (PI), and resistance index (RI) were recorded. The expression levels of GATA3 and CD34 in breast cancer tissues and adjacent tissues were detected by immunohistochemistry.Results:Compared with adjacent normal tissues, the proportion of low expression of GATA3 in breast cancer tissues was significantly increased, the difference was statistically significant ( χ2=9.673, P=0.002), and the proportion of high expression of CD34 was significantly increased, the difference was statistically significant ( χ2=37.104, P<0.001). The Emax, Emin, PI and RI in breast cancer patients with high GATA3 expression were significantly lower than those with low GATA3 expression, and the difference was statistically significant ( t was 4.136, 4.005, 5.387 and 4.103, respectively, P<0.05). The Emax, Emean, Emin, PI and RI of breast cancer patients with high expression of CD34 were significantly higher than those with low expression, with statistical significance ( t was 4.553, 4.037, 5.491, 3.894 and 5.273, respectively, P<0.05). Multivariate binary Logistic regression analysis showed that Emax, PI and RI were independent risk factors for low expression of GATA3 in breast cancer tissues, and the difference was statistically significant ( P<0.05). Emax, Emean, PI and RI were independent risk factors for high expression of CD34 in breast cancer tissues. The difference was statistically significant ( P<0.05). The sensitivity and specificity of ultrasound shear wave elastography combined with blood flow quantitative parameters were 78.7% and 81.7% for evaluating the low expression of GATA3 in breast cancer tissue, and 81.5% and 76.6% for evaluating the high expression of CD34 in breast cancer tissue, respectively. Conclusion:Ultrasonic shear wave elastic imaging and blood flow quantitative parameters are related to the expression levels of GATA3 and CD34 in breast cancer tissue, and have certain evaluation value.